The Mitochondria Marker Antibody Panel contains a curated set of antibodies targeting proteins localized to different mitochondrial sub-compartments. This panel enables comprehensive analysis of mitochondrial mass, health, and function, including markers for the outer membrane (TOM20), inner membrane (COX IV), and matrix (HSP60, SOD2).
Buffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Application Dilute:
Western Blot 1:500 - 1:1000, Immunochemistry 1:100 - 1:300, Optimal concentrations and conditions for each application should be determined by the user.
Cytochrome C antibody Western blot analysis of cell extracts using Cytochrome C orb1294348 at 1/1000 dilution.
VDAC1 antibody Western blot analysis of cell extracts using VDAC1 orb1294402 at 1/1000 dilution.
BAX antibody Western blot analysis of cell extracts using BAX orb1294359 at 1/1000 dilution.
AIF monoclonal antibody Western blot analysis of cell extracts using AIF monoclonal orb1973126 at 1/1000 dilution.
GAPDH antibody Western blot analysis of cell extracts using GAPDH antibody orb1294414 at 1/5000 dilution.
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