Caspase-1 p20 Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting CASP1. This antibody is suitable for FC, IF. It exhibits reactivity with Human, Mouse, Rat samples.
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Target:
CASP1
Application Dilute:
Flow-Cyt=1µg/Test, IF=1:100-500
Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-CASP1 antibody (orb318090), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CASP1 antibody (orb318090), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: U937. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (orb318090), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
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