Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Target:
BIRC3
Application Dilute:
WB: 1:1000, IF/ICC: 1:10-50, IHC-P: 1:10-50
orb32106
BIRC3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the BIRC3 antibody detected the BIRC3 protein (arrow).
Western blot analysis of BIRC3 (arrow) using rabbit polyclonal BIRC3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC3 gene.
Confocal immunofluorescent analysis of BIRC3 Antibody (N-term) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
BIRC3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIRC3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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