Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Target:
Raf1
Application Dilute:
WB: 1:1000, FACS: 1:10-50, IHC-P: 1:10-50
orb32409
Mouse Raf1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the Raf1 antibody detected the Raf1 protein (arrow).
Mouse Raf1 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35 ug/lane). This demonstrates the Raf1 antibody detected the Raf1 protein (arrow).
Mouse Raf1 Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Mouse Raf1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Raf1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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