ND6 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB325568
Article Name: ND6 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB325568
Supplier Catalog Number: orb325568
Alternative Catalog Number: BYT-ORB325568-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Human
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ND6
Conjugation: Unconjugated
Alternative Names: anti MTND6 antibody
Rabbit polyclonal antibody to ND6
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 19kDa
NCBI: 536854
UniProt: P03923
Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence: Synthetic peptide located within the following region: DGVVVVVNFNSVGSWMIYEGEGSGLIREDPIGAGALYDYGRWLVVVTGWT
Target: ND6
Sample Tissue: Human DLD1 Whole Cell, Antibody Dilution: 3 ug/mL.
Sample Tissue: Human HT1080 Whole Cell, Antibody Dilution: 1 ug/mL.
Sample Tissue: Human Lung Tumor, Antibody Dilution: 1 ug/mL.
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
WB Suggested Anti-ND6 Antibody Titration: 0.2-1 ug/mL, Positive Control: Human Lung.