Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Target:
EGR1
Application Dilute:
WB: 1:1000, FACS: 1:10-50, IF/ICC: 1:10-50
orb34304
Immunofluorescense analysis of Hela cell using EGR1 antibody (primary antibody dilution at: 1:10-50)
Western blot analysis of EGR1 Antibody (N-term) in mouse NIH-3T3 tissue lysates (35 ug/lane). EGR1 (arrow) was detected using the purified Pab.
EGR1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the EGR1 antibody detected the EGR1 protein (arrow).
Confocal immunofluorescent analysis of EGR1 Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
EGR1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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