Preservative: 0.01% (w/v) Sodium Azide, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Rat
Purity:
Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c). No reaction was observed against anti-Rat IgG F(ab)2 or anti-Papain.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Rat. Application Notes: Rat IgG F(c) Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments
B cell development was reconstituted in the Crescendo Mouse. (a) Flow cytometric analysis of bone marrow cells stained with anti-B220 antibody (horizontal axis) and antibodies to differentiation markers (vertical axis), CD117, CD43, Ly51, CD25, IgM-Fc (TKO & wt) or IgG-Fc (Crescendo Mouse), (b) photograph of spleens from wild type mouse, TKO mouse and Crescendo Mouse, (c) sandwich ELISA detecting serum Ig Fcgamma chains in TKO (circles), wild type (squares) and the Crescendo Mouse (triangles). Rat Fc fragments (p/n orb346338).
SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab, 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-Page of Rat IgG F(c). Lane 1: Rat Fc - Non-reduced. Lane 2: Rat Fc - Reduced. Load: 1.0 µg per lane. Predicted/Observed size: 25 kDa, 25 kDa for Reduced Fc. Other band(s): None.
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