Preservative: None. Stabilizer: 10% (v/v) Glycerol, Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10% glycerol
Source:
Human
Purity:
Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1, Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
Form:
Liquid
Application Dilute:
ELISA: 50ng, WB: 50ng
Application Notes:
Biological Origin: Human. Application Notes: Human AKT1 (phosphatase treated) recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT1 in immunological assays. Akt1 recombinant protein is dephosphorylated and in an inactive state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized
SDS-PAGE of AKT1 (phosphatase treated) Human Recombinant Protein. Lane 1: AKT1, unreduced. Lane 2: prestained MW markers. Lane 3: AKT1, reduced. Load: 1 µg per lane. Predicted/Observed size: 56 kDa, ~56 kDa for AKT1. Other band(s): none.
Western Blot of AKT1 (phosphatase treated) Human Recombinant Protein. Lane 1: SuperSignal MW markers. Lane2: AKT1. Load: 50 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for 1hour at room temperature. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 1 hour at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637), 1 hour at room temperature. Predicted/Observed size: 56kDa, 56kDa for AKT1. Other band(s): none
Western Blot of Rabbit AKT Antibodies. Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant: orb346473. Lane 3: AKT1, phosphatase-treated: orb346472. Lane 4: AKT1, mutant T308A/S473A: orb346474. Lane 5: AKT2, recombinant: orb346475. Lane 6: AKT2, phosphatase-treated: orb346470. Lane 7: AKT3, recombinant: orb346476. Lane 8: AKT3, phosphatase-treated: orb346471. Load: 50 ng per lane. Blot A: orb345379 Anti-Akt pT308 used at 1:2270, Blot B: orb750474 Anti-Akt used 1:1000.
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