Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Target:
PKM
Application Dilute:
WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
Western blot analysis of PKM2 (arrow) using rabbit polyclonal PKM2 Antibody (N-term E131). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).
PKM2 (N-term E131) Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PKM2 (N-term E131) Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PKM2 (N-term E131) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Western blot analysis of 293 cell lysateusing PKM2 antibody (primary antibody dilution at: 1:1000)
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