Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Target:
TIMP3
Application Dilute:
WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50
Western blot analysis of TIMP3 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). TIMP3 (arrow) was detected using the purified Pab.
TIMP3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TIMP3 Antibody (Center) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse NIH-3T3 cell line lysate. TIMP3 Antibody (Center) (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
orb39816
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