A synthetic peptide corresponding to a sequence at the N-terminus of human Relaxin 1, which shares 45.2% amino acid (aa) sequence identity with mouse Relaxin 1.
Conjugation:
Unconjugated
Alternative Names:
Prorelaxin H1, Relaxin B chain, Relaxin A chain, RLN1
Anti-Relaxin 1/RLN1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Prorelaxin H1 [Cleaved into: Relaxin B chain, Relaxin A chain]
Application Dilute:
Western blot, 0.1-0.5µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml
WB analysis of Relaxin 1 using anti-Relaxin 1 antibody.Lane 1:rat PC-12 cell.
IHC analysis of Relaxin 1 using anti-Relaxin 1 antibody. Relaxin 1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-Relaxin 1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Relaxin 1 using anti-Relaxin 1 antibody. Relaxin 1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-Relaxin 1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Relaxin 1 using anti-Relaxin 1 antibody. Relaxin 1 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-Relaxin 1 Antibody overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of Relaxin 1 using anti-Relaxin 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Relaxin 1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Relaxin 1 at approximately 21KD. The expected band size for Relaxin 1 is at 21KD.
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