Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 26.0 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human M-CSF, His Tag (Cat. No. orb402822) captured on CM5 Chip via anti-His antibody can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. orb257372) with an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).
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