Solubility: It is recommended to reconstitute the lyophilized sRANKL in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 50ng/ml shown in ''Corning Osteo Assay Surface 24 Well Plates with Transwell Permeable Supports- A Useful Tool for Co-Culture Studies by Rebecca M. Wood and Mark Rothenber, corresponding to a specific activity of 20,000Units/mg. Application Notes: Cytokines And Growth Factors
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