SOAT2 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB4332
- Images (6)
| Article Name: | SOAT2 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB4332 |
| Supplier Catalog Number: | orb4332 |
| Alternative Catalog Number: | BYT-ORB4332-50,BYT-ORB4332-100,BYT-ORB4332-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human ACAT2 (331-430/522aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ACACT2, ACAT2, ARGP2, SOAT2_HUMAN, SOAT2, Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT-2), Cholesterol acyltransferase 2, 2.3.1.26, |
| SOAT2 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 60 kDa |
| UniProt: | O75908 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SOAT2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (orb4332) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (orb4332) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (orb4332) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (orb4332) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOAT2) Polyclonal Antibody, Unconjugated (orb4332) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human K562 cell lysates, Primary: Anti-SOAT2 (orb4332) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 61 kDa. |
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