Phospho-Bad (Ser134) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB4671
- Images (7)
| Article Name: | Phospho-Bad (Ser134) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB4671 |
| Supplier Catalog Number: | orb4671 |
| Alternative Catalog Number: | BYT-ORB4671-50,BYT-ORB4671-100,BYT-ORB4671-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser134 PK(p-S)AG |
| Conjugation: | Unconjugated |
| Alternative Names: | Bad (p-S134), p-BAD, phospho-BAD, BBC2, BCL2L8, Bad_v1, Bad_v2, BAD_HUMAN, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8 (Bcl2-L-8), Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6, BAD_MOUSE, BAD_RAT, |
| Phospho-Bad (Ser134) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 18 kDa |
| UniProt: | Q92934 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | BAD |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test |
| Application Notes: | Modification: Phosphorylated |
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Blank control (Black line): Jurkat (Black). Primary Antibody (green line): Rabbit Anti-Phospho-Bad (Ser134) antibody (orb4671), dilution: 3 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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IHC-P analysis of Human kidney tissue using Bad Ser134 Polyclonal Antibody at 1:400 dilution. |
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