0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
PROM1
Application Dilute:
WB=1:500-2000, Flow-Cyt=1µg/Test
Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-CD133 antibody (orb526485), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 2: Lovo (Human) Cell Lysate at 30 ug Lane 3: SW480 (Human) Cell Lysate at 30 ug Lane 4: Uterus (Mouse) Lysate at 40 ug Primary: Anti-CD133 (orb526485) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 115 kD.
Sample: SP2/0 Cell (Mouse) Lysate at 40 ug, Colon carcinoma (Human) Lysate at 40 ug, Primary: Anti-CD133 (orb526485) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 95 kD, Observed band size: 95/120 kD.
The blue histogram is unstained cells (HepG 2). The Orange histogram is cells stained with Rabbit IgG/FITC (orb525556), The green histogram is cells stained with Rabbit Anti-CD133/FITC Conjugated antibody. Isotype control: Cell lines treated with Rabbit IgG/FITC (orb525556) instead of the primary antibody to confirm that primary antibody binding is 2 µg/5 µg/1 µg in 100 µl 1X PBS containing 0.5% BSA.
The blue histogram is unstained cells (HepG 2). The Orange histogram is cells stained with Rabbit IgG/PE, The green histogram is cells stained with Rabbit Anti-CD133/PE Conjugated antibody. Isotype control: Cell lines treated with Rabbit IgG/PE instead of the primary antibody to confirm that primary antibody binding is specific. 2 µg/5 µg/10 µg in 100 µl 1X PBS containing 0.5% BSA.
Flow cytometry of Mouse kidney.Primary Antibody (green line): Rabbi
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