E2F3 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB526587
Article Name: E2F3 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB526587
Supplier Catalog Number: orb526587
Alternative Catalog Number: BYT-ORB526587-50,BYT-ORB526587-100,BYT-ORB526587-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human E2F3 (361-460/465aa)
Conjugation: Unconjugated
Alternative Names: E2F-3, E2F3_HUMAN, E2F3, KIAA0075,
E2F3 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 49 kDa
UniProt: O00716
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: E2F3
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated (orb526587) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated (orb526587) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated (orb526587) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-E2F3 (orb526587) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 50 kD.
IHC-P analysis of rat liver using E2F3 Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of mouse liver using E2F3 Polyclonal Antibody at 1:200 dilution.



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