Collagen II Mouse Monoclonal Antibody, Clone: [9H12], Unconjugated

Catalog Number: BYT-ORB526771
Article Name: Collagen II Mouse Monoclonal Antibody, Clone: [9H12], Unconjugated
Biozol Catalog Number: BYT-ORB526771
Supplier Catalog Number: orb526771
Alternative Catalog Number: BYT-ORB526771-50,BYT-ORB526771-100,BYT-ORB526771-200
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: IF, IHC-Fr, IHC-P
Species Reactivity: Human
Immunogen: KLH conjugated synthetic peptide derived from human Collagen II (1301-1400/1487aa)
Conjugation: Unconjugated
Alternative Names: ACG2, ANFH, ANFH1, AOM, COL11A3, EDMMD, LCPD, OSCDP, PLSDT, SEDC, SEDSTN, SEMDSTWK, SMDALG, STL1, VPED, Col2, Col2a, Col2a-1, Del1, Dmm, Lpk, M100413, Rgsc413, Rgsc856, CG2A1A, COLLII, CO2A1_HUMAN, COL2A1, Alpha-1 type II collagen, CO2A1_MOUSE, CO2A1_RAT,
Collagen II Mouse Monoclonal Antibody
Clonality: Monoclonal
Concentration: 1mg/ml
Clone Designation: [9H12]
Molecular Weight: 142 kDa
UniProt: P02458
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: COL2A1
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen II) Monoclonal Antibody, Unconjugated (orb526771) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen II) Monoclonal Antibody, Unconjugated (ascites of orb526771 9H12) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen II) Monoclonal Antibody, Unconjugated (orb526771) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen II) Monoclonal Antibody, Unconjugated (orb526771) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat fetal bone), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen II) Monoclonal Antibody, Unconjugated (orb526771) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen II) Monoclonal Antibody, Unconjugated (orb526771) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.




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