5-Ethynyl-2-deoxycytidine (5-EdC), CAS [[69075-47-4]]
Biozol Catalog Number:
BYT-ORB532418
Supplier Catalog Number:
orb532418
Alternative Catalog Number:
BYT-ORB532418-10
Manufacturer:
Biorbyt
Category:
Biochemikalien
Alternative Names:
5-Ethynyl-2-deoxycytidine
Ethynyl-labeled deoxycytidine (5-EdC) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) to measure de novo DNA syn-thesis during the S-phase of the cell cycle. 5-EdC is cell-permeabel and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (Azides of Biotin) for subsequent purification tasks or a fluorescent group (Azides of fluorescent dyes) for subse-quent microscopic imaging.
