5-(3-Aminoallyl)-2-deoxyuridine-5-triphosphate, labeled with ATTO 425, Triethylammonium salt
Aminoallyl-dUTP-XX-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-ATTO425/80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-XX-ATTO425/70-50% dTTP (Nick Translation).
