5-(3-Aminoallyl)-2-deoxyuridine-5-triphosphate, labeled with Cy5, Triethylammonium salt
Aminoallyl-dUTP-XX-Cy5 is recommended for direct enzymatic label-ing of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-Cy5/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-Cy5/80-70% dTTP.
