5-Propargylamino-2-deoxycytidine-5-triphosphate, labeled with Cy3, Triethylammonium salt
5-Propargylamino-dCTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy3/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy3/70-50% dCTP.
