Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP.
filtered solution (30 kDa) in 10 mM Tris-HCl, Color: colorless to slightly yellow, pH: 7.5 0.5
Formula:
C32H54N9O15P3S
Application Notes:
Application Notes: Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data, Primer Extension with Klenow fragment. Spectroscopic Propertie: lambdamax 266 nm, epsilon 16.2 L mmol-1 cm-1 (Tris-HCl, pH 7.5)
Structural formula of Biotin-14-dATP.
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