Phospho-HSP27 (Ser15) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB5483
Article Name: Phospho-HSP27 (Ser15) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB5483
Supplier Catalog Number: orb5483
Alternative Catalog Number: BYT-ORB5483-50,BYT-ORB5483-100,BYT-ORB5483-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSP27 around the phosphorylation site of Ser15 GP(p-S)WD
Conjugation: Unconjugated
Alternative Names: HSPB1 | HSP27 (p-S15), p-HSP27, phospho-HSP27, CMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27, 27kDa, HSPB1_CANLF, HSPB1, Heat shock 27 kDa protein (HSP 27), HSPB1_HUMAN, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27 (SRP27), HSPB1_MOUSE, Growth-related 25 kDa protein, Heat shock 25 kDa protein (HSP 25), p25, HSPB1_RAT,
Phospho-HSP27 (Ser15) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 23 kDa
UniProt: P04792
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: HSPB1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg /Test
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of human kidney tissue using HSP27 (phospho-Ser15) antibody.
Immunohistochemical staining of human gastric carcinoma tissue using HSP27 (phospho-Ser15) antibody.
Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 20 min at -20C). Primary Antibody (green line): Rabbit Anti-Phospho-HSP27 (Ser15) antibody (orb5483), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-HSP27 (Ser15)) polyclonal Antibody, Unconjugated (orb5483) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-HSP27 (Ser15)) Polyclonal Antibody, Unconjugated (orb5483) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-HSP27 (Ser15) (orb5483) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.



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