Cytokeratin 2e Recombinant Rabbit Monoclonal Antibody, Clone: [2F7], Unconjugated
Catalog Number:
BYT-ORB559076
- Images (7)
| Article Name: | Cytokeratin 2e Recombinant Rabbit Monoclonal Antibody, Clone: [2F7], Unconjugated |
| Biozol Catalog Number: | BYT-ORB559076 |
| Supplier Catalog Number: | orb559076 |
| Alternative Catalog Number: | BYT-ORB559076-50,BYT-ORB559076-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant human Cytokeratin 2e protein, around 350-500aa |
| Conjugation: | Unconjugated |
| Alternative Names: | CK-2e, K2e, KRT2A, KRT2E, KRTE, K22E_HUMAN, KRT2, Cytokeratin-2e (CK-2e), Epithelial keratin-2e, Keratin-2 epidermis, Keratin-2e (K2e), Type-II keratin Kb2, |
| Cytokeratin 2e Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [2F7] |
| Molecular Weight: | 70 kDa |
| UniProt: | P35908 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | KRT2 |
| Application Dilute: | WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, ELISA=1:5000-10000 |
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Paraformaldehyde-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 2e) Monoclonal Antibody, Unconjugated (orb559076) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 2e) Polyclonal Antibody, Unconjugated (orb559076) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 2e) Polyclonal Antibody, Unconjugated (orb559076) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 2e) Monoclonal Antibody, Unconjugated (orb559076) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 2e) Polyclonal Antibody, Unconjugated (orb559076) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: human skin tissue lysates, Primary: Anti-Cytokeratin 2e (orb559076) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution, Predicted band size: 70 kD, Observed band size: 65 kD. |
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IHC-P analysis of human esophagus using Cytokeratin 2e Monoclonal Antibody at 1:50 dilution. |
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