Phospho-EGFR (Ser695) Recombinant Rabbit Monoclonal Antibody, Clone: [7B12], Unconjugated

Catalog Number: BYT-ORB559114
Article Name: Phospho-EGFR (Ser695) Recombinant Rabbit Monoclonal Antibody, Clone: [7B12], Unconjugated
Biozol Catalog Number: BYT-ORB559114
Supplier Catalog Number: orb559114
Alternative Catalog Number: BYT-ORB559114-50,BYT-ORB559114-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ICC, IF, IHC-Fr, IHC-P
Species Reactivity: Human
Immunogen: A synthesized peptide derived from human EGFR around the phosphorylation site of S695 TP-pS-GE
Conjugation: Unconjugated
Alternative Names: EGFR | ERBB1/HER1 (p-S695), p-ERBB1/HER1, phospho-ERBB1/HER1, ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA, 9030024J15Rik, Errb1, Wa5, wa-2, wa2, ErbB-1, EGFR_HUMAN, EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, 2.7.10.1, EGFR_MOUSE,
Phospho-EGFR (Ser695) Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [7B12]
Molecular Weight: 170 kDa
UniProt: P00533
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: EGFR
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-EGFR (Ser695)) Monoclonal Antibody, Unconjugated (orb559114) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-EGFR (Ser695)) Monoclonal Antibody, Unconjugated (orb559114) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-EGFR (Ser695)) Monoclonal Antibody, Unconjugated (orb559114) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-EGFR (Ser695)) Monoclonal Antibody, Unconjugated (orb559114) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-EGFR (Ser695)) Monoclonal Antibody, Unconjugated (orb559114) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-EGFR (Ser695)) Monoclonal Antibody, Unconjugated (orb559114) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
IHC-P analysis of rat stomach using phospho-EGFR Ser695 Monoclonal Antibody at 1:200 dilution.