0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
STAT2
Application Dilute:
WB=1:500-1000, ICC/IF=1:50-200
25 ug total protein per lane of various lysates probed with STAT2 monoclonal antibody, unconjugated (orb559149) at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Immunocytochemistry analysis of A431 cells labeling STAT2 with Rabbit anti-STAT2 antibody (orb559149) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37C, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-STAT2 antibody (orb559149) at 1/200 dilution in 2% negative goat serum overnight at 4C. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of A549 cells labeling STAT2 with Rabbit anti-STAT2 antibody (orb559149) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37C, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-STAT2 antibody (orb559149) at 1/200 dilution in 2% negative goat serum overnight at 4C. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of SW480 cells labeling STAT2 with Rabbit anti-STAT2 antibody (orb559149) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37C, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-STAT2 antibody (orb559149) at 1/200 dilution in 2% negative goat serum overnight at 4C. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-STAT2 antibody (orb559149) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb559149) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-STAT2 antibody (orb559149) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb559149) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse skin cancer tissue with Rabbit anti-STAT2 antibody (orb559149) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb559149) at 1/400 dilution for 1 hour at room temperature. The
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