Phospho-BTK (Tyr223) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB5651
- Images (7)
| Article Name: | Phospho-BTK (Tyr223) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB5651 |
| Supplier Catalog Number: | orb5651 |
| Alternative Catalog Number: | BYT-ORB5651-50,BYT-ORB5651-100,BYT-ORB5651-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rabbit |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Btk around the phosphorylation site of Tyr223 AL(p-Y)DY |
| Conjugation: | Unconjugated |
| Alternative Names: | BTK (p-Y223), p-BTK, phospho-BTK, AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA, xid, BTK_HUMAN, BTK, Agammaglobulinemia tyrosine kinase (ATK), B-cell progenitor kinase (BPK), Bruton tyrosine kinase, 2.7.10.2, BTK_MOUSE, Kinase EMB, |
| Phospho-BTK (Tyr223) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 76 kDa |
| UniProt: | Q06187 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | BTK |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test, ELISA=1:5000-10000 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-BTK (Tyr223)) Polyclonal Antibody, Unconjugated (orb5651) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-BTK (Tyr223)) Polyclonal Antibody, Unconjugated (orb5651) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-BTK (Tyr223)) Polyclonal Antibody, Unconjugated (orb5651) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
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phosphopeptide, non phosphopeptide. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-BTK (Tyr223) Polyclonal Antibody, Unconjugated (orb5651) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-BTK (Tyr223) Polyclonal Antibody, Unconjugated (orb5651) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-BTK (Tyr223) Antibody (orb5651) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |
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