Application Notes: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain. Each alpha and beta chain has a calculated MW of 74.1 kDa (beta chain) and 114.8 kDa (alpha chain). The protein migrates as 70 kDa and 110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Rat Complement C5, His Tag (Cat. No. orb570266) at 2 µg/mL (100 µL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-12.5 ng/mL (QC tested).
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