Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Loaded Mouse IL-2 R alpha, His tag (Cat. No. orb570281) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 21.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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