Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 83.5 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag with an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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