Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Loaded Human IL-6 R alpha, Fc Tag (Cat. No. orb570285) on Protein A Biosensor, can bind Human IL-6, premium grade (Cat. No. orb257601) with an affinity constant of 35.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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