NFKB p65 Recombinant Rabbit Monoclonal Antibody, Clone: [3G1], Unconjugated
Catalog Number:
BYT-ORB608066
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| Article Name: | NFKB p65 Recombinant Rabbit Monoclonal Antibody, Clone: [3G1], Unconjugated |
| Biozol Catalog Number: | BYT-ORB608066 |
| Supplier Catalog Number: | orb608066 |
| Alternative Catalog Number: | BYT-ORB608066-100,BYT-ORB608066-25,BYT-ORB608066-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human NF-kB p65 (500-551/551aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | AIF3BL3, CMCU, NFKB3, p65, p65 NF-kappa B, p65 NFkB, NFkB, nos2, TF65_HUMAN, RELA, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, TF65_MOUSE, |
| NFKB p65 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [3G1] |
| Molecular Weight: | 61 kDa |
| UniProt: | Q04206 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | RELA |
| Application Dilute: | WB=1:1000-5000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:100-200, IF=1:100-200, Flow-Cyt=1:50-100 |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (orb608066) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (orb608066) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (orb608066) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (orb608066) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (orb608066) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (orb608066) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (orb608066) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: MCF-7 (Human) Cell Lysate at 30 ug, Molt-4 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Spleen (Mouse) Lysate at 40 ug, Lung (Mouse) Lysate at 40 ug, Primary: Anti-NFKB p65 (orb608066) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 63 kD. |
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