Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody, Clone: [3G3], Unconjugated
Catalog Number:
BYT-ORB612206
- Images (7)
| Article Name: | Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody, Clone: [3G3], Unconjugated |
| Biozol Catalog Number: | BYT-ORB612206 |
| Supplier Catalog Number: | orb612206 |
| Alternative Catalog Number: | BYT-ORB612206-50,BYT-ORB612206-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | A synthesized peptide derived from human Tryptase 1 (10-275/275aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | TPS1, TPS2, TPSB1, TPSB2, Tryptase-2, MMCP-7, Mcp-7, Mcp7, Mcpt7, rMCP-7, TRYB1_HUMAN, TPSAB1, Tryptase-1, Tryptase I, Tryptase alpha-1, 3.4.21.59, TRYB1_MOUSE, Mast cell protease 7 (mMCP-7), Tryptase alpha/beta-1, TRYB1_RAT, Mast cell protease 7 (rMCP-7), Tryptase, skin, TRYB2_HUMAN, Tryptase II, |
| Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [3G3] |
| Molecular Weight: | 23 kDa |
| UniProt: | Q15661 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TPSAB1 |
| Application Dilute: | WB=1:500-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000 |
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Paraformaldehyde-fixed, paraffin embedded (human Abdominal skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human coion), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Mouse Stomach tissue lysates, Primary: Anti-Mast Cell Tryptase (orb612206) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 32 kDa. |
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Western blot:Lane 1:human skin,Primary:Anti-Mast Cell Tryptase at 1:500 dilution. |
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