TFRC Recombinant Rabbit Monoclonal Antibody, Clone: [8G6], Unconjugated
Catalog Number:
BYT-ORB612221
- Images (8)
| Article Name: | TFRC Recombinant Rabbit Monoclonal Antibody, Clone: [8G6], Unconjugated |
| Biozol Catalog Number: | BYT-ORB612221 |
| Supplier Catalog Number: | orb612221 |
| Alternative Catalog Number: | BYT-ORB612221-25,BYT-ORB612221-50,BYT-ORB612221-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, WB |
| Species Reactivity: | Human, Rat |
| Immunogen: | A synthesized peptide derived from human Transferrin receptor (2-50/760aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90, 2610028K12Rik, E430033M20Rik, Mtvr1, TFR1_HUMAN, TFRC, TFR1_MOUSE, TFR1_RAT, |
| TFRC Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [8G6] |
| Molecular Weight: | 95 kDa |
| UniProt: | P02786 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TFRC |
| Application Dilute: | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1µg/Test |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (orb612221) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (orb612221) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD71 Transferrin receptor) Monoclonal Antibody, Unconjugated (orb612221) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD71 Transferrin receptor) Monoclonal Antibody, Unconjugated (orb612221) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD71 Transferrin receptor) Monoclonal Antibody, Unconjugated (orb612221) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD71 Transferrin receptor) Monoclonal Antibody, Unconjugated (orb612221) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD71 Transferrin receptor) Monoclonal Antibody, Unconjugated (orb612221) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: HT1080 (Human) Cell Lysate at 30 ug, Lane 4: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD71/Transferrin receptor (orb612221) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95 kD, Observed band size: 100 kD. |
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