Histone H3 (Di Methyl K36) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6155
- Images (8)
| Article Name: | Histone H3 (Di Methyl K36) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6155 |
| Supplier Catalog Number: | orb6155 |
| Alternative Catalog Number: | BYT-ORB6155-50,BYT-ORB6155-100,BYT-ORB6155-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Di Methyl K36 GV(Di Methyl-K)KP |
| Conjugation: | Unconjugated |
| Alternative Names: | H3C1 | Histone H3 (me2-K36), me2-Histone H3, di-methyl-Histone H3, H3K36me2, me2-H3, H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3/b, H3C1, H3FB, HIST1H3D, H3.1, H3/c, H3FC, HIST1H3C, H3/d, H3FD, HIST1H3E, H3.f, H3/f, H3FF, HIST1H3I, H3/h, H3FH, HIST1H3G, H3/j, H3FJ, HIST1H3J, H3/k, H3F1K, H3FK, HIST1H3H, H3/l, H3FL, HIST1H3B, H3/i, H3FI, HIST1H3F, H3.2-221, H3c13, H3c14, H3c15, Hist1, H3-143, H3-b, H3-53, H3-f, H3-291, H3-H, H3.1-221, H3 histone, Hist1h2ail, Hist1h2ail1, Hist2h3c, Hist3h3, H31_HUMAN, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FC HIST1H3C, H33_HUMAN, H3-3A, H3-3B, H3.3A, H3F3, H3F3A, H3.3B, H3F3B, H31T_HUMAN, H3-4, H3/t, H3t, H3/g, Histone H3.4, H3FT, H3C_HUMAN, H3-5, Histone H3.5, H3F3C, H32_HUMAN, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, HIST2H3A, H3F2, H3FM, HIST2H3D, H31_MOUSE, H3a, H3g, H3.1-291, H3h, H3.1-I, H3i, H32_MOUSE, H3-clustered histone 2, H3-clustered histone 3, H3-clustered histone 4, H3-clustered histone 6, H3-clustered histone 7, H3.2, H3b, H3f, H3.2-616, Hist2h3b, H3.2-615, Hist2h3c1, Hist2h3ca1, H3.2-614, Hist2h3c2, Hist2h3ca2, H31_RAT, |
| Histone H3 (Di Methyl K36) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 15 kDa |
| UniProt: | P68431 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | H3C1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg /Test |
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Flow cytometric analysis of Hela Cell using Di Methyl-Histone H3 (K36) antibody. |
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Histone H3 (Di Methyl K36) antibody (orb6155), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (orb6155) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (Di Methyl K36)) Polyclonal Antibody, Unconjugated (orb6155) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (Di Methyl K36)) Polyclonal Antibody, Unconjugated (orb6155) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (orb6155) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (Di Methyl K36)) Polyclonal Antibody, Unconjugated (orb6155) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: SiHa (Human) Cell Lysate at 30 ug, Lane 2: U-2OS (Human) Cell Lysate at 30 ug, Primary: Anti-Histone H3 (Di Methyl K36) (orb6155) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 17 kD. |
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