Phospho-IRAK1 (Ser376) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6221
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| Article Name: | Phospho-IRAK1 (Ser376) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6221 |
| Supplier Catalog Number: | orb6221 |
| Alternative Catalog Number: | BYT-ORB6221-100,BYT-ORB6221-200,BYT-ORB6221-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Ser376 QS(p-S)TV |
| Conjugation: | Unconjugated |
| Alternative Names: | IRAK1 (p-S376), p-IRAK1, phospho-IRAK1, IRAK, pelle, IRAK-1, IRAK1-S, IRAK1b, Il1rak, Plpk, mPLK, RGD1563841, IRAK1_HUMAN, IRAK1, 2.7.11.1, IRAK1_MOUSE, Pelle-like protein kinase (mPLK), |
| Phospho-IRAK1 (Ser376) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 78 kDa |
| UniProt: | P51617 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | Irak1 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test |
| Application Notes: | Modification: Phosphorylated |
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Western blot analysis of A549 lysates using IRAK1 (phospho-Ser376) antibody |
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Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-IRAK1 (Ser376) antibody (orb6221), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-IRAK1 (Ser376)) polyclonal Antibody, Unconjugated (orb6221) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRAK1 (Ser376)) Polyclonal Antibody, Unconjugated (orb6221) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRAK1 (Ser376)) Polyclonal Antibody, Unconjugated (orb6221) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: human gastric cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-IRAK1 (Ser376) Polyclonal Antibody, Unconjugated (orb6221) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Human lung cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-IRAK1 (Ser376) Polyclonal Antibody, Unconjugated (orb6221) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-FRA1 (Ser265) Polyclonal Antibody, Unconjugated 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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