Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6223
- Images (7)
| Article Name: | Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6223 |
| Supplier Catalog Number: | orb6223 |
| Alternative Catalog Number: | BYT-ORB6223-100,BYT-ORB6223-200,BYT-ORB6223-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr387 RG(p-T)LA |
| Conjugation: | Unconjugated |
| Alternative Names: | IRAK1 (p-T387), p-IRAK1, phospho-IRAK1, IRAK, pelle, IRAK-1, IRAK1-S, IRAK1b, Il1rak, Plpk, mPLK, RGD1563841, IRAK1_HUMAN, IRAK1, 2.7.11.1, IRAK1_MOUSE, Pelle-like protein kinase (mPLK), |
| Phospho-IRAK1 (Thr387) Rabbit Polyclonal Antibody |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2µg /Test, ELISA=1:5000-10000 |
| Application Notes: | Modification: Phosphorylated |
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Blank control (blue): RSC96 cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-IRAK1 (Thr387) antibody (orb6223), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRAK1 (Thr387)) Polyclonal Antibody, Unconjugated (orb6223) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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phosphopeptide, non phosphopeptide. |
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Sample: Lane 1: Liver (Mouse) Cell Lysate at 30 ug, Lane 2: Colon carcinoma (Human) Lysate at 30 ug, Primary: Anti-Phospho-IRAK1 (Thr387) (orb6223) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 70kD, Observed band size: 70kD. |
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Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-IRAK1 (Thr387) Polyclonal Antibody, Unconjugated (orb6223) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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