ITGAV Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6245
- Images (7)
| Article Name: | ITGAV Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6245 |
| Supplier Catalog Number: | orb6245 |
| Alternative Catalog Number: | BYT-ORB6245-50,BYT-ORB6245-100,BYT-ORB6245-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Integrin alpha V (51-150/1048aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CD51, MSK8, VNRA, VTNR, ITAV_HUMAN, ITGAV, Vitronectin receptor, Vitronectin receptor subunit alpha, |
| ITGAV Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 95/113 kDa |
| UniProt: | P06756 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | ITGAV |
| Application Dilute: | WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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WB analysis of mouse lung tissue using Integrin alpha V antibody (dilution at 1:5000) |
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Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4C). Primary Antibody (green line): Rabbit Anti-CD51 antibody (orb6245), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. |
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Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-CD51 antibody (orb6245), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ITGAV) Polyclonal Antibody, Unconjugated (orb6245) at 1:500 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ITGAV) Polyclonal Antibody, Unconjugated (orb6245) at 1:500 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-CD51 (orb6245) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125-140 kD, Observed band size: 140 kD. |
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U-2OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (CD51) polyclonal Antibody, Unconjugated (orb6245) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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