Immunohistochemical staining of rat brain tissue using LPL protein antibody.
Immunohistochemical staining of rat brain tissue using LPL protein antibody.
Blank control (blue line): raji (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37C and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Lipoprotein lipase antibody (orb6325), dilution: 0.2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (orb6325) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (orb6325) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HL-60 cell lysates, Primary: Anti-Lipoprotein lipase (orb6325) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kDa, Observed band size: 60 kDa.
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