Flow cytometric analysis of U937 cell using CD91 antibody.
Immunohistochemical staining of Mouse brain tissue using CD91 antibody.
Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min). Primary Antibody: Rabbit Anti-LRP1 antibody (orb6328), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-LRP1 (orb6328) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.
Tissue/Cell: Mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-LRP1 Polyclonal Antibody, Unconjugated (orb6328) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Mouse lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-LRP1 Polyclonal Antibody, Unconjugated (orb6328) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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