Phospho-DAXX (Ser517) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6351
Article Name: Phospho-DAXX (Ser517) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6351
Supplier Catalog Number: orb6351
Alternative Catalog Number: BYT-ORB6351-50,BYT-ORB6351-100,BYT-ORB6351-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human DAXX around the phosphorylation site of Ser517 SR(p-S)SG
Conjugation: Unconjugated
Alternative Names: DAXX (p-S517), p-DAXX, phospho-DAXX, BING2, DAP6, EAP1, SMIM40, DAXX_HUMAN, DAXX, Daxx (hDaxx), ETS1-associated protein 1 (EAP1), Fas death domain-associated protein, DAXX_MOUSE, DAXX_RAT,
Phospho-DAXX (Ser517) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 81 kDa
UniProt: Q9UER7
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: DAXX
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of rat brain tissue using DAXX (phospho-Ser517) antibody.
Immunohistochemical staining of mouse kidney tissue using DAXX (phospho-Ser517) antibody.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-DAXX (Ser517) antibody (orb6351), dilution: 3 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (rat breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-DAXX (Ser517)) Polyclonal Antibody, Unconjugated (orb6351) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: K562 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-DAXX (Ser517) (orb6351) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD.
Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-DAXX (Ser517) Polyclonal Antibody, Unconjugated (orb6351) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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