MSH6 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6442
Article Name: MSH6 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6442
Supplier Catalog Number: orb6442
Alternative Catalog Number: BYT-ORB6442-50,BYT-ORB6442-100,BYT-ORB6442-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human MSH6 (812-910/1360aa)
Conjugation: Unconjugated
Alternative Names: GTBP, GTMBP, HNPCC5, HSAP, LYNCH5, MMRCS3, MSH-6, p160, MSH6_HUMAN, MSH6, hMSH6, G/T mismatch-binding protein (GTBP | GTMBP), MutS protein homolog 6, MutS-alpha 160 kDa subunit (p160), MSH6_MOUSE,
MSH6 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 153 kDa
UniProt: P52701
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MSH6
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat colon tissue using MSH6 antibody.
Immunohistochemical staining of human lung cancer tissue using MSH6 antibody.
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MSH6) Polyclonal Antibody, Unconjugated (orb6442) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MSH6) Polyclonal Antibody, Unconjugated (orb6442) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: 293T (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Primary: Anti-MSH6 (orb6442) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 153 kD, Observed band size: 121 kD.
Sample: U87MG (Human) Cell Lysate at 30 ug, Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-MSH6 (orb6442) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 153 kD, Observed band size: 120/121 kD.



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