Phospho-MSK1 (Ser212) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6445
Article Name: Phospho-MSK1 (Ser212) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6445
Supplier Catalog Number: orb6445
Alternative Catalog Number: BYT-ORB6445-50,BYT-ORB6445-100,BYT-ORB6445-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthesised phosphopeptide derived from human MSK1 around the phosphorylation site of Ser212 AY(p-S)FC
Conjugation: Unconjugated
Alternative Names: RPS6KA5 | MSK1 (p-S212), p-MSK1, phospho-MSK1, MSK1, MSPK1, RLPK, 3110005L17Rik, 6330404E13Rik, RLSK, S6K-alpha-5, KS6A5_HUMAN, RPS6KA5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase (RSKL), 2.7.11.1, KS6A5_MOUSE, RSK-like protein kinase (RLSK),
Phospho-MSK1 (Ser212) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 90 kDa
UniProt: O75582
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: RPS6KA5
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of Rat lung tissue using MSK1 (phospho-Ser212) antibody.
Immunohistochemical staining of rat brain tissue using MSK1 (phospho-Ser212) antibody.
Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-Phospho-MSK1 (Ser212) antibody (orb6445), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-MSK1 (Ser212)) polyclonal Antibody, Unconjugated (orb6445) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Sample: Lane 1: Human 293T cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HUVEC cell lysates, Lane 5: Human A549 cell lysates, Lane 6: Human MDA-MB-231 cell lysates, Primary: Anti-Phospho-MSK1 (Ser212) (orb6445) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kDa, Observed band size: 85 kDa.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-MSK1 Polyclonal Antibody, Unconjugated (orb6445) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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