Phospho-mTOR (Ser2448) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6452
- Images (6)
| Article Name: | Phospho-mTOR (Ser2448) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6452 |
| Supplier Catalog Number: | orb6452 |
| Alternative Catalog Number: | BYT-ORB6452-50,BYT-ORB6452-100,BYT-ORB6452-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human mTOR around the phosphorylation site of Ser2448 TD(p-S)YS |
| Conjugation: | Unconjugated |
| Alternative Names: | mTOR (p-S2448), p-mTOR, phospho-mTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS, 2610315D21Rik, flat, MTOR_HUMAN, MTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin (mTOR), Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Tyrosine-protein kinase mTOR, 2.7.11.1, MTOR_MOUSE, Rapamycin target protein 1 (RAPT1), MTOR_RAT, |
| Phospho-mTOR (Ser2448) Rabbit Polyclonal Antibody |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg /test |
| Application Notes: | Modification: Phosphorylated |
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Phospho-mTOR (Ser2448) antibody (orb6452), dilution: 1 µg/10 6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-mTOR (Ser2448)) Polyclonal Antibody, Unconjugated (orb6452) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-mTOR (Ser2448)) Polyclonal Antibody, Unconjugated (orb6452) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody, Unconjugated (orb6452) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-mTOR Polyclonal Antibody, Unconjugated (orb6452) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-mTOR Polyclonal Antibody, Unconjugated (orb6452) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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