Phospho-NFKB1 (Ser337) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6516
- Images (7)
| Article Name: | Phospho-NFKB1 (Ser337) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6516 |
| Supplier Catalog Number: | orb6516 |
| Alternative Catalog Number: | BYT-ORB6516-50,BYT-ORB6516-100,BYT-ORB6516-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human NF KappaB p105 around the phosphorylation site of Ser337 RK(p-S)DL |
| Conjugation: | Unconjugated |
| Alternative Names: | NFKB1 | NFkB p105 (p-S337), p-NFkB p105, phospho-NFkB p105, CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB, NF-kappaB1, p105, p50, p50/p105, NFKB1_HUMAN, NFKB1, DNA-binding factor KBF1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, NFKB1_MOUSE, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1_RAT, |
| Phospho-NFKB1 (Ser337) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 48/105 kDa |
| UniProt: | P19838 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | NFKB1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
| Application Notes: | Modification: Phosphorylated |
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Flow cytometric analysis of A549 cell using NFKB1 (phospho-Ser337) antibody. |
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Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-NFKB1 (Ser337) antibody (orb6516), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (orb6516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (orb6516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (orb6516) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-NFKB1 (Ser337) (orb6516) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48/105 kD, Observed band size: 48kD. |
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Sample: U87-MG (Human) Lysate at 30 ug, MCF7 (Human) Lysate at 30 ug, Primary: Anti-Phospho-NFKB1 (Ser337) (orb6516) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48/105 kD, Observed band size: 48/105 kD. |
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