Phospho-MKK3 (Ser218) + MKK6 (Ser207) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6579
Article Name: Phospho-MKK3 (Ser218) + MKK6 (Ser207) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6579
Supplier Catalog Number: orb6579
Alternative Catalog Number: BYT-ORB6579-50,BYT-ORB6579-100,BYT-ORB6579-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthesised phosphopeptide derived from human MKK3 around the phosphorylation site of Ser218 VD(p-S)VA
Conjugation: Unconjugated
Alternative Names: MEK3 & MEK6 (p-T222), p-MEK3 & MEK6, phospho-MEK3 & MEK6, MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2, MAPKK 3, MKK3b, MP2K3_HUMAN, MAP2K3, MAP kinase kinase 3, MAPK/ERK kinase 3 (MEK 3), Stress-activated protein kinase kinase 2 (SAPK kinase 2 | SAPKK-2 | SAPKK2), 2.7.12.2, SKK2, MP2K3_MOUSE,
Phospho-MKK3 (Ser218) + MKK6 (Ser207) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 39 kDa
UniProt: P46734
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MAP2K3
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of rat brain tissue using MKK6 (phospho-Ser207) antibody.
Immunohistochemical staining of human lung cancer tissue using MKK6 (phospho-Ser207) antibody.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (orb6579) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (orb6579) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (orb6579) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-MKK3 (Ser218) + MKK6 (Ser207) (orb6579) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 40 kD.
Sample: Muscle (Rat) Lysate at 40 ug, Primary: Anti-Phospho-MKK3 (Ser218) + MKK6 (Ser207) (orb6579) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 40 kD.



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