Phospho-P53 (Thr18) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6601
- Images (7)
| Article Name: | Phospho-P53 (Thr18) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6601 |
| Supplier Catalog Number: | orb6601 |
| Alternative Catalog Number: | BYT-ORB6601-50,BYT-ORB6601-100,BYT-ORB6601-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Thr18 QE(p-T)FS |
| Conjugation: | Unconjugated |
| Alternative Names: | TP53 | p53 (p-T18), p-p53, phospho-p53, BCC7, BMFS5, LFS1, P53, TRP53, Tp53, bbl, bfy, bhy, p44, P53_BOVIN, Tumor suppressor p53, P53_HUMAN, Antigen NY-CO-13, Phosphoprotein p53, P53_MOUSE, P53_RAT, |
| Phospho-P53 (Thr18) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 43 kDa |
| UniProt: | P04637 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TP53 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg /test |
| Application Notes: | Modification: Phosphorylated |
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-P53 (Thr18) antibody (orb6601), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-P53 (Thr18)) Polyclonal Antibody, Unconjugated (orb6601) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-P53 (Thr18)) Polyclonal Antibody, Unconjugated (orb6601) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-P53 (Thr18)) Polyclonal Antibody, Unconjugated (orb6601) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: Molt-4 (Human) Cell Lysate at 30 ug, Lane 4: Esophagus (Mouse) Lysate at 40 ug, Lane 5: Esophagus (Rat) Lysate at 40 ug, Lane 6: Urinary bladder (Mouse) Lysate at 40 ug, Lane 7: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-phospho-P53 (Thr18) (orb6601) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD. |
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IHC-P analysis of mouse bladder using phospho-P53 Thr18 Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of Rat bladder using phospho-P53 Thr18 Polyclonal Antibody at 1:200 dilution. |
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