Phospho-Paxillin (Tyr88) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6650
- Images (8)
| Article Name: | Phospho-Paxillin (Tyr88) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6650 |
| Supplier Catalog Number: | orb6650 |
| Alternative Catalog Number: | BYT-ORB6650-50,BYT-ORB6650-100,BYT-ORB6650-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr88 PV(p-Y)GS |
| Conjugation: | Unconjugated |
| Alternative Names: | PXN | Paxillin (p-Y88), p-Paxillin, phospho-Paxillin, Pax, PAXI_HUMAN, PXN, PAXI_MOUSE, PAXI_RAT, |
| Phospho-Paxillin (Tyr88) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 68 kDa |
| UniProt: | P49023 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PXN |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (orb6650) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (orb6650) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (orb6650) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (orb6650) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (orb6650) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Paxillin (Tyr88) (orb6650) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD. |
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Sample: Embryo (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Paxillin (Tyr88) (orb6650) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD. |
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IHC-P analysis of rat testis tissue using P-Paxillin Tyr88 Polyclonal Antibody at 1:400 dilution. |
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