Phospho-AKT1 (Ser129) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6781
Article Name: Phospho-AKT1 (Ser129) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6781
Supplier Catalog Number: orb6781
Alternative Catalog Number: BYT-ORB6781-50,BYT-ORB6781-100,BYT-ORB6781-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Akt1 around the phosphorylation site of Ser129 DN(p-S)GA
Conjugation: Unconjugated
Alternative Names: AKT1 (p-S129), p-AKT1, phospho-AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, LTR-akt, PKB/Akt, PKBalpha, O57513_CHICK, akt1, RAC-PK-alpha, 2.7.11.1, AKT1_HUMAN, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, AKT1_MOUSE, AKT1 kinase, Thymoma viral proto-oncogene, AKT1_RAT,
Phospho-AKT1 (Ser129) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 56 kDa
UniProt: P31749
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: AKT1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of human breast carcinoma tissue using AKT (phospho-Ser129) antibody.
Immunohistochemical staining of human mammary cancer tissue using AKT (phospho-Ser129) antibody.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-phospho-AKT1 (Ser129) antibody (orb6781), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser129)) Polyclonal Antibody, Unconjugated (orb6781) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser129)) Polyclonal Antibody, Unconjugated (orb6781) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser129)) Polyclonal Antibody, Unconjugated (orb6781) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Sample: Lane 1: SiHa (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AKT1 (Ser129) (orb6781) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 5961 kD, Observed band size: 59 kD.



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